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Anti-Phospho-Myosin Light Chain 2 (Thr18, Ser19) Antibody (9L462) is a Rabbit antibody targeting Phospho-Myosin Light Chain 2 (Thr18, Ser19). Anti-Phospho-Myosin Light Chain 2 (Thr18, Ser19) Antibody (9L462) can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μL | $81 | 7-10 days | |
100 μL | $233 | 7-10 days |
Description | Antibody Type: Rabbit Monoclonal Application: WB Reactivity: Human |
Alias | p-Myosin Light Chain 2 (Thr18, Ser19), p-Myosin Light Chain 2 (T18, S19), Phospho-Myosin Light Chain 2 (T18, S19), MYRL2, Myosin Light Chain 2 (pThr18, Ser19), Myosin Light Chain 2 (pT18, S19), MRLC1, MMIHS4, MLC-2C, MLC2, LC20 |
Ig Type | Rabbit IgG |
Clone | 9L462 |
Reactivity | Human;
Predicted to React with:This antbody also react with Human Phospho-Myosin Light Chain 2 (Thr18), which correspond to Phospho-Myosin Light Chain 2 (Thr19) of P24844-1 in UniProt in ELISA.; Species predicted to react based on 100% sequence homology: Mouse, Cynomolgus, Bovine, Pig |
Verified Activity | 1. Western blot analysis of extracts from serum-starved Hela, untreated (line A) or treated with CalyculinA (100nM, 30min)(line B), using Phospho-Myosin Light Chain 2 (Thr18, Ser19) rabbit monoclonal Antibody at 1:10000 dilution (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution (lower). 2. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with Calyculin A (100nM, 30min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Myosin Light Chain 2 (Thr18, Ser19) rabbit monoclonal Antibody at 1:10000 dilution. (Validation Experiment) |
Application | WB |
Recommended Dose | WB: 1:10000-1:100000 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the residues around (Thr18, Ser19) of Human Phospho-Myosin Light Chain 2. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: residues around (Thr18, Ser19) of Human Phospho-Myosin Light Chain 2 |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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