Anti-Phospho-MyoD1 (Ser200) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MyoD1 (Ser200). Anti-Phospho-MyoD1 (Ser200) Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Pig,Cow)

1. Blank control: K562.
Primary Antibody (green line): Rabbit Anti-phospho-MyoD1 (Ser200) antibody (TMAB-11021)
Dilution:1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Tongue tissue lysates
Lane 3: Mouse NIH/3T3 cell lysates
Primary: Anti-phospho-MyoD1 (Ser200) (TMAB-11021) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 47 kDa

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human MyoD1 around the phosphorylation site of Ser200

Human

MYOD1

Myoblast determination protein 1

Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity).