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Anti-Phospho-MYL9 (Ser20) Polyclonal Antibody
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Catalog No. TMAB-01460
Anti-Phospho-MYL9 (Ser20) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MYL9 (Ser20). Anti-Phospho-MYL9 (Ser20) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-MYL9 (Ser20) Polyclonal Antibody
Anti-Phospho-MYL9 (Ser20) Polyclonal Antibody
😃Good
Catalog No. TMAB-01460
Anti-Phospho-MYL9 (Ser20) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MYL9 (Ser20). Anti-Phospho-MYL9 (Ser20) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-MYL9 (Ser20) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MYL9 (Ser20). Anti-Phospho-MYL9 (Ser20) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-phospho-MLc (Ser20) Polyclonal Antibody, Unconjugated (TMAB-01460) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-phospho-MLc (Ser20) Polyclonal Antibody, Unconjugated (TMAB-01460) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Tissue/cell: human kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-phospho-MLc (Ser20) Polyclonal Antibody, Unconjugated (TMAB-01460) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus. 4. Sample: Kidney (Mouse) Lysate at 40 μg Primary: Anti-Myosin light chain (phospho S20) (TMAB-01460) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm<br>Cytoskeleton |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthesised phosphopeptide: human MYL9 around the phosphorylation site of Ser20 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cytoskeleton,Signal Transduction,Motor Proteins,Myosin,ECM |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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