Anti-Phospho-MYL (Ser19) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MYL (Ser19). Anti-Phospho-MYL (Ser19) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Tissue/cell: smooth muscle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Phospho-MYl (Ser19) Polyclonal Antibody, Unconjugated (TMAB-11019) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: Heart (Rat) Lysate at 40 μg
Primary: Anti-Phospho-MYl (Ser19) (TMAB-11019) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 18 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human MYL2 around the phosphorylation site of Ser19

Human

MYL2

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.