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Anti-Phospho-MYC (Thr58) Antibody (7T717)
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Catalog No. TMAC-03203
Alias p-MYC (Thr58), p-MYC (T58), MYC (p-Thr58), MYC (p-T58)
Anti-Phospho-MYC (Thr58) Antibody (7T717) is a Rabbit antibody targeting Phospho-MYC (Thr58). Anti-Phospho-MYC (Thr58) Antibody (7T717) can be used in FCM,ICC/IF,WB.
Anti-Phospho-MYC (Thr58) Antibody (7T717)
Anti-Phospho-MYC (Thr58) Antibody (7T717)
😃Good
Catalog No. TMAC-03203Alias p-MYC (Thr58), p-MYC (T58), MYC (p-Thr58), MYC (p-T58)
Anti-Phospho-MYC (Thr58) Antibody (7T717) is a Rabbit antibody targeting Phospho-MYC (Thr58). Anti-Phospho-MYC (Thr58) Antibody (7T717) can be used in FCM,ICC/IF,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $298 | 7-10 days | 7-10 days | |
| 100 μL | $498 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Phospho-MYC (Thr58) Antibody (7T717) is a Rabbit antibody targeting Phospho-MYC (Thr58). Anti-Phospho-MYC (Thr58) Antibody (7T717) can be used in FCM,ICC/IF,WB. |
| Synonyms | p-MYC (Thr58), p-MYC (T58), MYC (p-Thr58), MYC (p-T58) |
| Ig Type | IgG |
| Clone | 7T717 |
| Reactivity | Human |
| Verified Activity | 1. ICC staining phospho-c-Myc (T58) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 2. ICC staining phospho-c-Myc (T58) in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. Flow cytometric analysis of MCF-7 cells with phospho-c-Myc (T58) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.    |
| Application | |
| Recommended Dose | WB: 1:1000; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | A synthesized phosphopeptide: human c-Myc around the phosphorylation site of Thr58 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 50 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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