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Anti-Phospho-MKK3/6 (Ser218, 207) Polyclonal Antibody
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Catalog No. TMAB-01453
Anti-Phospho-MKK3/6 (Ser218, 207) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MKK3/6 (Ser218, 207). Anti-Phospho-MKK3/6 (Ser218, 207) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-MKK3/6 (Ser218, 207) Polyclonal Antibody
Anti-Phospho-MKK3/6 (Ser218, 207) Polyclonal Antibody
😃Good
Catalog No. TMAB-01453
Anti-Phospho-MKK3/6 (Ser218, 207) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MKK3/6 (Ser218, 207). Anti-Phospho-MKK3/6 (Ser218, 207) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-MKK3 (Ser218) + MKK6 (Ser207)) Polyclonal Antibody, Unconjugated (TMAB-01453) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Muscle (Mouse) Lysate at 40 μg Primary: Anti-Phospho-MKK3 (Ser218) + MKK6 (Ser207) (TMAB-01453) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 40 kDa 3. Sample: Muscle (Rat) Lysate at 40 μg Primary: Anti-Phospho-MKK3 (Ser218) + MKK6 (Ser207) (TMAB-01453) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 40 kDa 4. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Phospho-MKK3 (Ser218) + MKK6 (Ser207)) Polyclonal Antibody, Unconjugated (TMAB-01453) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Phospho-MKK3 (Ser218) + MKK6 (Ser207)) Polyclonal Antibody, Unconjugated (TMAB-01453) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm; cytoskeleton. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthesised phosphopeptide: human MKK3 around the phosphorylation site of Ser218 |
| Antigen Species | Human |
| Gene ID | 5606 |
| Uniprot ID | |
| Biology Area | MAPK pathway,Other factors,TLR Signaling,MAPK Pathway,Tyrosine Kinases |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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