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Anti-Phospho-MEK5 (Ser137) Polyclonal Antibody

Catalog No. TMAB-10991 Copy Product Info
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Anti-Phospho-MEK5 (Ser137) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MEK5 (Ser137). Anti-Phospho-MEK5 (Ser137) Polyclonal Antibody can be used in WB.

Anti-Phospho-MEK5 (Ser137) Polyclonal Antibody

Copy Product Info
🥰Excellent
Catalog No. TMAB-10991

Anti-Phospho-MEK5 (Ser137) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MEK5 (Ser137). Anti-Phospho-MEK5 (Ser137) Polyclonal Antibody can be used in WB.

Anti-Phospho-MEK5
(Ser137) Polyclonal Antibody
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2207-10 days7-10 days
100 μL$3717-10 days7-10 days
200 μL$5297-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Phospho-MEK5 (Ser137) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MEK5 (Ser137). Anti-Phospho-MEK5 (Ser137) Polyclonal Antibody can be used in WB.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Sample: Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-phospho-MEK5 (Ser137) (TMAB-10991) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 50 kD
2. Sample: MDA-MB-231 Cell (Human) Lysate at 40 μg
Primary: Anti-phospho-MEK5 (Ser137) (TMAB-10991) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 50 kD
Application
Recommended Dose
WB: 1:500-2000
Antibody Type
Polyclonal
Host SpeciesRabbit
Tissue SpecificityExpressed in many adult tissues. Abundant in heart and skeletal muscle.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundMEK5 is a dual specificity protein kinase belonging to thr Ser/Thr protein kinase family, (MAP kinase kinase family). It is activated by phosphorylation on Ser/Thr by MAP kinase kinases and interacts specifically with ERK5, and not with another MAP kinase like P38. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). It is not phosphorylated by RAFA, RAFB or RAFC and it may interact with GTPases such as CDC42. The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. MEK5 is expressed in many adult tissues and is most abundant in heart and skeletal muscle.
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human MEK5 around the phosphorylation site of Ser137
Antigen Species
Human
Gene Name
MAP2K5
Gene ID
Protein Name
Dual specificity mitogen-activated protein kinase kinase 5
Uniprot ID
Function
Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.
Chemical Properties
Molecular WeightTheoretical: 49 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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