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Anti-Phospho-MEK1 (Thr292) Antibody (6W742)
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Catalog No. TMAB-10983
Anti-Phospho-MEK1 (Thr292) Antibody (6W742) is a Rabbit antibody targeting Phospho-MEK1 (Thr292). Anti-Phospho-MEK1 (Thr292) Antibody (6W742) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-MEK1 (Thr292) Antibody (6W742)
Anti-Phospho-MEK1 (Thr292) Antibody (6W742)
Copy Product Info😃Good
Catalog No. TMAB-10983
Anti-Phospho-MEK1 (Thr292) Antibody (6W742) is a Rabbit antibody targeting Phospho-MEK1 (Thr292). Anti-Phospho-MEK1 (Thr292) Antibody (6W742) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $262 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-MEK1 (Thr292) Antibody (6W742) is a Rabbit antibody targeting Phospho-MEK1 (Thr292). Anti-Phospho-MEK1 (Thr292) Antibody (6W742) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Clone | 6W742 |
| Reactivity | Human |
| Verified Activity | 1. Jurkat (H) cells were treated with or without calyculin A (100 nM) for 30 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-MEK1 (Thr292) monoclonal antibody, unconjugated (TMAB-10983) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-MEK1 (Thr292) Monoclonal Antibody, Unconjugated (TMAB-10983) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-MEK1 (Thr292) Monoclonal Antibody, Unconjugated (TMAB-10983) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-MEK1 (Thr292) Monoclonal Antibody, Unconjugated (TMAB-10983) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-MEK1 (Thr292) Monoclonal Antibody, Unconjugated (TMAB-10983) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules. |
| Immunogen | A synthesized peptide: human MEK1 around the phosphorylation site of T292 |
| Antigen Species | Human |
| Gene Name | Map2k1 |
| Gene ID | |
| Protein Name | Dual specificity mitogen-activated protein kinase kinase 1 |
| Uniprot ID |
| Molecular Weight | Theoretical: 43 kDa. Actual: 45 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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