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Anti-Phospho-MARK4 (Ser423) Polyclonal Antibody

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Catalog No. TMAB-10951

Anti-Phospho-MARK4 (Ser423) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MARK4 (Ser423). Anti-Phospho-MARK4 (Ser423) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Phospho-MARK4 (Ser423) Polyclonal Antibody

Anti-Phospho-MARK4 (Ser423) Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-10951
Anti-Phospho-MARK4 (Ser423) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MARK4 (Ser423). Anti-Phospho-MARK4 (Ser423) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5287-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Phospho-MARK4 (Ser423) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MARK4 (Ser423). Anti-Phospho-MARK4 (Ser423) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Cow,Horse)
Verified Activity
1. Sample: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-MARK4 (Ser423) (TMAB-10951) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 75 kD
Observed band size: 77 kD
2. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-MARK4 (Ser423)) Polyclonal Antibody, Unconjugated (TMAB-10951) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a member of the microtubule affinity-regulating kinase family. These protein kinases phosphorylate microtubule-associated proteins and regulate the transition between stable and dynamic microtubules. The encoded protein is associated with the centrosome throughout mitosis and may be involved in cell cycle control. Expression of this gene is a potential marker for cancer, and the encoded protein may also play a role in Alzheimer's disease. Pseudogenes of this gene are located on both the short and long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010].
Antigen Details
Immunogen
KLH conjugated synthesised phosphopeptide: human MARK4 around the phosphorylation site of Ser423
Antigen Species
Human
Gene Name
MARK4
Gene ID
Protein Name
MAP/microtubule affinity-regulating kinase 4
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 75 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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