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Anti-Phospho-MARCKS (Ser170) Polyclonal Antibody 2
Catalog No. TMAB-10949 Copy Product Info
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Anti-Phospho-MARCKS (Ser170) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-MARCKS (Ser170). Anti-Phospho-MARCKS (Ser170) Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.
Anti-Phospho-MARCKS (Ser170) Polyclonal Antibody 2
Copy Product Info🥰Excellent
Catalog No. TMAB-10949
Anti-Phospho-MARCKS (Ser170) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-MARCKS (Ser170). Anti-Phospho-MARCKS (Ser170) Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF.
Anti-Phospho-MARCKS
(Ser170) Polyclonal Antibody 2
(Ser170) Polyclonal Antibody 2
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-Phospho-MARCKS (Ser170) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-MARCKS (Ser170). Anti-Phospho-MARCKS (Ser170) Polyclonal Antibody 2 can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Chicken,Dog,Cow) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-MARCKS (Ser170)) Polyclonal Antibody, Unconjugated (TMAB-10949) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-MARCKS (Ser170)) Polyclonal Antibody, Unconjugated (TMAB-10949) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton (Probable). Membrane; Lipid-anchor. |
| Tissue Specificity | Highest levels found in spleen and brain. Intermediate levels seen in thymus, ovary, lung and heart. Very low levels seen in kidney, skeletal muscle and liver. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human MARCKS around the phosphorylation site of Ser170 |
| Antigen Species | Human |
| Gene Name | MARCKS |
| Gene ID | |
| Protein Name | Myristoylated alanine-rich C-kinase substrate |
| Uniprot ID | |
| Function | MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. |
| Molecular Weight | Theoretical: 31 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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