Anti-Phospho-MARCKS (Ser152+Ser156) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MARCKS (Ser152+Ser156). Anti-Phospho-MARCKS (Ser152+Ser156) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Chicken,Dog,Cow,Rabbit)

1. Sample:
Spleen (Mouse) Lysate at 40 μg
Cerebrum (Mouse) Lysate at 40 μg
Cerebellum (Mouse) Lysate at 40 μg
Primary: Anti-Phospho-MARCKS (Ser152+Ser156) (TMAB-10948) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-MARCKS (Ser152+Ser156)) Polyclonal Antibody, Unconjugated (TMAB-10948) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Rat Cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-MARCKS (Ser152+Ser156)) Polyclonal Antibody, Unconjugated (TMAB-10948) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthesised phosphopeptide: rat MARCKS around the phosphorylation site of Ser152/156 or Ser159/163(human)

Human

MARCKS

Myristoylated alanine-rich C-kinase substrate

MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.