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Anti-Phospho-MARCKS (Ser152+Ser156) Polyclonal Antibody
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Catalog No. TMAB-10948
Anti-Phospho-MARCKS (Ser152+Ser156) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MARCKS (Ser152+Ser156). Anti-Phospho-MARCKS (Ser152+Ser156) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-MARCKS (Ser152+Ser156) Polyclonal Antibody
Anti-Phospho-MARCKS (Ser152+Ser156) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-10948
Anti-Phospho-MARCKS (Ser152+Ser156) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MARCKS (Ser152+Ser156). Anti-Phospho-MARCKS (Ser152+Ser156) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-MARCKS (Ser152+Ser156) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MARCKS (Ser152+Ser156). Anti-Phospho-MARCKS (Ser152+Ser156) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Chicken,Dog,Cow,Rabbit) |
| Verified Activity | 1. Sample: Spleen (Mouse) Lysate at 40 μg Cerebrum (Mouse) Lysate at 40 μg Cerebellum (Mouse) Lysate at 40 μg Primary: Anti-Phospho-MARCKS (Ser152+Ser156) (TMAB-10948) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-MARCKS (Ser152+Ser156)) Polyclonal Antibody, Unconjugated (TMAB-10948) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Rat Cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-MARCKS (Ser152+Ser156)) Polyclonal Antibody, Unconjugated (TMAB-10948) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton (Probable). Membrane; Lipid-anchor. |
| Tissue Specificity | Highest levels found in spleen and brain. Intermediate levels seen in thymus, ovary, lung and heart. Very low levels seen in kidney, skeletal muscle and liver. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin. |
| Immunogen | KLH conjugated synthesised phosphopeptide: rat MARCKS around the phosphorylation site of Ser152/156 or Ser159/163(human) |
| Antigen Species | Human |
| Gene Name | MARCKS |
| Gene ID | |
| Protein Name | Myristoylated alanine-rich C-kinase substrate |
| Uniprot ID | |
| Function | MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. |
| Molecular Weight | Theoretical: 30 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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