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Anti-Phospho-MAPKAPK-2 (Thr334) Antibody (5R815) is a Rabbit antibody targeting Phospho-MAPKAPK-2 (Thr334). Anti-Phospho-MAPKAPK-2 (Thr334) Antibody (5R815) can be used in WB.
Description | Anti-Phospho-MAPKAPK-2 (Thr334) Antibody (5R815) is a Rabbit antibody targeting Phospho-MAPKAPK-2 (Thr334). Anti-Phospho-MAPKAPK-2 (Thr334) Antibody (5R815) can be used in WB. |
Ig Type | Monoclonal Rabbit IgG |
Clone | 5R815 |
Reactivity | Human; Predicted to React with:Species predicted to react based on 100% sequence homology: Mouse, Rat, Bovine, Rabbit |
Verified Activity | 1. Western blot analysis of extracts from serum-starved A431, untreated(line A) or treated with calyculin A (100mM, 30min; +)(line B), using Phospho-MAPKAPK-2 (Thr334) rabbit monoclonal Antibody at 1:200 dilution (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution (lower). 2. Western blot analysis of extracts from serum-starved A431, untreated (line A); treated with calyculin A (100mM, 30min; +) (line B); treated with calyculin A and λ-phosphatase (line C) using Phospho-MAPKAPK-2 (Thr334) rabbit monoclonal Antibody at 1:200 dilution. (Validation Experiment) 3. Western blot analysis of extracts from serum-starved A431, untreated (line A); treated with calyculin A (100mM, 30min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-MAPKAPK-2 (Thr334) rabbit monoclonal Antibody at 1:200 dilution. (Validation Experiment) |
Application | WB |
Recommended Dose | WB: 1:500-1:2000 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the residues around (Thr334) of Human Phospho-MAPKAPK-2. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide corresponding to the residues around (Thr334) of Human Phospho-MAPKAPK-2 |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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