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Anti-Phospho-MAPK3/ERK2 (Tyr204) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MAPK3/ERK2 (Tyr204). Anti-Phospho-MAPK3/ERK2 (Tyr204) Polyclonal Antibody can be used in WB,IHC,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $216 | 7-10 days | |
100 μL | $316 | 7-10 days |
Description | Anti-Phospho-MAPK3/ERK2 (Tyr204) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MAPK3/ERK2 (Tyr204). Anti-Phospho-MAPK3/ERK2 (Tyr204) Polyclonal Antibody can be used in WB,IHC,IF. |
Synonyms | p-MAPK3/ERK2 (Y204), p-MAPK3/ERK2 (Tyr204), MAPK3/ERK2 (p-Y204), MAPK3/ERK2 (p-Tyr204) |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204) Antibody TMAC-02966 (left) or the same antibody preincubated with blocking peptide (right). 2. Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using p44/42 MAP Kinase (Phospho-Tyr204) Antibody TMAC-02966. 3. Immunofluorescence staining of methanol-fixed Hela cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204) Antibody TMAC-02966. 4. Western blot analysis of various lysates using p44/42 MAP Kinase (Phospho-Tyr204) Antibody at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (L3012) at 1:10000 dilution. Lysates/proteins: 25μg per lane. |
Application | |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Polyclonal Antibody |
Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Appearance | Liquid |
Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 |
Conjucates | Unconjugated |
Others Formats | Phospho |
Immunogen | Peptide sequence around phosphorylation site of tyrosine 204 (T-E-Y(p)-V-A) derived from Human p44/42 MAP Kinase |
Antigen Species | human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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