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Anti-Phospho-MAP4K4 (Ser801) Polyclonal Antibody
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Catalog No. TMAB-10938
Anti-Phospho-MAP4K4 (Ser801) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MAP4K4 (Ser801). Anti-Phospho-MAP4K4 (Ser801) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-MAP4K4 (Ser801) Polyclonal Antibody
Anti-Phospho-MAP4K4 (Ser801) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-10938
Anti-Phospho-MAP4K4 (Ser801) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MAP4K4 (Ser801). Anti-Phospho-MAP4K4 (Ser801) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-MAP4K4 (Ser801) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MAP4K4 (Ser801). Anti-Phospho-MAP4K4 (Ser801) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Chicken,Dog,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phosphor-MAP 4 k4) Polyclonal Antibody, Unconjugated (TMAB-10938) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 2. Sample: Hela Cell (Human) Lysate at 30 μg HepG2 Cell (Human) Lysate at 30 μg Primary: Anti-p-MAP 4 k4 (Ser801) (TMAB-10938) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 142 kD Observed band size: 135 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Tissue Specificity | Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human MAP4K4 around the phosphorylation site of Ser801 |
| Antigen Species | Human |
| Gene Name | MAP4K4 |
| Gene ID | |
| Protein Name | Mitogen-activated protein kinase kinase kinase kinase 4 |
| Uniprot ID | |
| Function | Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322. |
| Molecular Weight | Theoretical: 142 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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