Anti-Phospho-MAP4K4 (Ser801) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MAP4K4 (Ser801). Anti-Phospho-MAP4K4 (Ser801) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Chicken,Dog,Horse,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phosphor-MAP 4 k4) Polyclonal Antibody, Unconjugated (TMAB-10938) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Sample:
Hela Cell (Human) Lysate at 30 μg
HepG2 Cell (Human) Lysate at 30 μg
Primary: Anti-p-MAP 4 k4 (Ser801) (TMAB-10938) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 142 kD
Observed band size: 135 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human MAP4K4 around the phosphorylation site of Ser801

Human

MAP4K4

Mitogen-activated protein kinase kinase kinase kinase 4

Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.