Anti-Phospho-MAP3K9+MAP3K10 (Thr312+Thr266) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MAP3K9+MAP3K10 (Thr312+Thr266). Anti-Phospho-MAP3K9+MAP3K10 (Thr312+Thr266) Polyclonal Antibody can be used in WB.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit)

1. Sample: MCF-7 (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-MAP3 k9+MAP3 k10 (Thr312+Thr266) (TMAB-10934) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121/105 kD
Observed band size: 121 kD
2. Sample: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-MAP3 k9+MAP3 k10 (Thr312+Thr266) (TMAB-10934) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121/105 kD
Observed band size: 121 kD

WB: 1:500-2000

Polyclonal

KLH conjugated synthesised phosphopeptide: human MAP3K9/MAP3K10 around the phosphorylation site of Thr312/Thr266

Human

MAP3K9

Mitogen-activated protein kinase kinase kinase 9

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.