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Anti-Phospho-MAP2 (Thr1613/1619) Polyclonal Antibody
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Catalog No. TMAB-10929
Anti-Phospho-MAP2 (Thr1613/1619) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MAP2 (Thr1613/1619). Anti-Phospho-MAP2 (Thr1613/1619) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-Phospho-MAP2 (Thr1613/1619) Polyclonal Antibody
Anti-Phospho-MAP2 (Thr1613/1619) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-10929
Anti-Phospho-MAP2 (Thr1613/1619) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MAP2 (Thr1613/1619). Anti-Phospho-MAP2 (Thr1613/1619) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-MAP2 (Thr1613/1619) Polyclonal Antibody is a Rabbit antibody targeting Phospho-MAP2 (Thr1613/1619). Anti-Phospho-MAP2 (Thr1613/1619) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (P-MAP2 (Thr1613,1619)) Polyclonal Antibody, Unconjugated (TMAB-10929) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Blank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-Phospho-MAP2 (Thr1613/1619) antibody Dilution: 1 μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg /test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. 3. Sample: Lane 1: SH-SY5Y (Human) Cell Lysate at 30 μg Lane 2: Large intestine (Mouse) Lysate at 40 μg Primary: Anti-Phospho-MAP2 (Thr1613/1619) (TMAB-10929) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 280/70-82 kD Observed band size: 75 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton (Probable). |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| Concentration | 1mg/ml |
| Research Background | MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton. |
| Immunogen | KLH conjugated synthesised phosphopeptide: human MAP2 around the phosphorylation site of Thr1613 and Thr1619 |
| Antigen Species | Human |
| Gene Name | MAP2 |
| Gene ID | |
| Protein Name | Microtubule-associated protein 2 |
| Uniprot ID | |
| Function | The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. |
| Molecular Weight | Theoretical: 70/201 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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