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Anti-Phospho-LIMK1 (Thr508) Polyclonal Antibody is a Rabbit antibody targeting Phospho-LIMK1 (Thr508). Anti-Phospho-LIMK1 (Thr508) Polyclonal Antibody can be used in WB,IHC,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $216 | 7-10 days | |
100 μL | $318 | 7-10 days |
Description | Anti-Phospho-LIMK1 (Thr508) Polyclonal Antibody is a Rabbit antibody targeting Phospho-LIMK1 (Thr508). Anti-Phospho-LIMK1 (Thr508) Polyclonal Antibody can be used in WB,IHC,IF. |
Synonyms | p-LIMK1 (Thr508), p-LIMK1 (T508), LIMK1 (p-Thr508), LIMK1 (p-T508) |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of extracts from UV-treated C2C12, PMA-treated Hela and anisomycin-treated 293 cells using LIMK1 (Phospho-Thr508) Antibody TMAC-02399. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK1 (Phospho-Thr508) Antibody TMAC-02399 (left) or the same antibody preincubated with blocking peptide (right). 3. Immunofluorescence staining of methanol-fixed Hela cells using LIMK1 (Phospho-Thr508) Antibody TMAC-02399. 4. Lane 1: Helatreated with PMA; Lane 2: Mouse brain tissue; Lane 3: Rat brain tissue; Lane 4: N-peptide blocked Helatreated with PMA; Lane 5: N-peptide blocked Mouse brain tissue; Lane 6: N-peptide blocked Rat brain tissue;Lane 7 : P-peptide blocked Helatreated with PMA; Lane 8: P-peptide blocked Mouse brain tissue; Lane 9: P-peptide blocked Rat brain tissue; Lysates/proteins at 40 μg per lane. Predicted band size: 72 kDa, Observed band size: 72 kDa. |
Application | |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Polyclonal Antibody |
Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Appearance | Liquid |
Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development. |
Conjucates | Unconjugated |
Others Formats | Phospho |
Immunogen | Peptide sequence around phosphorylation site of threonine 508 (R-Y-T(p)-V-V) derived from Human LIMK1 |
Antigen Species | human |
Uniprot ID |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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