Anti-Phospho-KLF5 (Ser275) Polyclonal Antibody is a Rabbit antibody targeting Phospho-KLF5 (Ser275). Anti-Phospho-KLF5 (Ser275) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Cow,Horse,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-KLF5 (Ser275)) Polyclonal Antibody, Unconjugated (TMAB-10896) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-KLF5 (Ser275)) Polyclonal Antibody, Unconjugated (TMAB-10896) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
3. Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human HT-1080 cell lysates
Lane 4: Human A549 cell lysates
Lane 5: Human A431 cell lysates
Primary: Anti-phospho-KLF5 (Ser275) (TMAB-10896) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 60 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthesised phosphopeptide: human KLF5 around the phosphorylation site of Ser275

Human

KLF5

Krueppel-like factor 5

Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.