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Anti-Phospho-JAK2 (Tyr221) Polyclonal Antibody

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Catalog No. TMAB-01442
Alias p-JAK2 (Y221), p-JAK2 (Tyr221), JAK2 (p-Y221), JAK2 (p-Tyr221)

Anti-Phospho-JAK2 (Tyr221) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JAK2 (Tyr221). Anti-Phospho-JAK2 (Tyr221) Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.

Anti-Phospho-JAK2 (Tyr221) Polyclonal Antibody

Anti-Phospho-JAK2 (Tyr221) Polyclonal Antibody

😃Good
Catalog No. TMAB-01442Alias p-JAK2 (Y221), p-JAK2 (Tyr221), JAK2 (p-Y221), JAK2 (p-Tyr221)
Anti-Phospho-JAK2 (Tyr221) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JAK2 (Tyr221). Anti-Phospho-JAK2 (Tyr221) Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3747-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-JAK2 (Tyr221) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JAK2 (Tyr221). Anti-Phospho-JAK2 (Tyr221) Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Aliasp-JAK2 (Y221), p-JAK2 (Tyr221), JAK2 (p-Y221), JAK2 (p-Tyr221)
Ig Type
IgG
Reactivity
Human,Zebrafish (predicted:Mouse,Rat,Dog,Pig,Horse)
Verified Activity
1. Blank control: HL-60
Primary Antibody (green line): Rabbit Anti-phospho-JAK2 (Tyr221) antibody (TMAB-01442)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-JAK2 (Tyr221)) polyclonal Antibody, Unconjugated (TMAB-01442) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
3. Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-JAK2 (Tyr221)) Polyclonal Antibody, Unconjugated (TMAB-01442) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
IHC-P,IHC-Fr,ICC/IF,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationEndomembrane system; Peripheral membrane protein (By similarity). Cytoplasm. Nucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthesised phosphopeptide: human JNK2 around the phosphorylation site of Tyr221
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cell cycle inhibitors,Kinases/phosphatases,Signal transducers,Cell Cycle Inhibitors,Kinases/Phosphatases,STATs,SARS Coronavirus,Tyrosine Kinases
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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