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Anti-Phospho-JAK2 (Tyr1007, 1008) Antibody (2X102) is a Rabbit antibody targeting Phospho-JAK2 (Tyr1007, 1008). Anti-Phospho-JAK2 (Tyr1007, 1008) Antibody (2X102) can be used in IHC-P,IHC-Fr,ICC/IF,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
25 μL | $155 | 7-10 days | |
50 μL | $272 | 7-10 days | |
100 μL | $489 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: IHC-P,IHC-Fr,ICC/IF,IF Reactivity: Human,Mouse,Rat |
Ig Type | IgG |
Clone | 2X102 |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Phospho-JAK2 (Y1007+Y1008)) monoclonal Antibody, Unconjugated (TMAB-01441) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 2. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-JAK2 (Tyr1007+Tyr1008) Monoclonal Antibody, Unconjugated (TMAB-01441) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-JAK2 (Tyr1007+Tyr1008) Monoclonal Antibody, Unconjugated (TMAB-01441) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-JAK2 (Tyr1007+Tyr1008) Monoclonal Antibody, Unconjugated (TMAB-01441) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-JAK2 (Tyr1007+Tyr1008) Monoclonal Antibody, Unconjugated (TMAB-01441) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-JAK2 (Tyr1007+Tyr1008) Monoclonal Antibody, Unconjugated (TMAB-01441) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Diffuse large B-cell lymphoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-JAK2 (Tyr1007+Tyr1008) Monoclonal Antibody, Unconjugated (TMAB-01441) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-JAK2 (Tyr1007+Tyr1008) Monoclonal Antibody, Unconjugated (TMAB-01441) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-JAK2 (Tyr1007+Tyr1008) Monoclonal Antibody, Unconjugated (TMAB-01441) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-JAK2 (Tyr1007+Tyr1008) Monoclonal Antibody, Unconjugated (TMAB-01441) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
Application | IHC-P,IHC-Fr,ICC/IF,IF |
Recommended Dose | IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Subcellular Localization | Endomembrane system; Peripheral membrane protein (By similarity). Cytoplasm. Nucleus. |
Construction | Recombinant Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008] |
Immunogen | A synthesized peptide: human JAK2 around the phosphorylation site of Y1007/Y1008 |
Antigen Species | Human |
Gene ID | 3717 |
Uniprot ID | |
Biology Area | Cell cycle inhibitors,Kinases/phosphatases,Signal transducers,Cell Cycle Inhibitors,Kinases/Phosphatases,STATs,SARS Coronavirus,Tyrosine Kinases |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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