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Anti-Phospho-JAK1 (Tyr1022) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JAK1 (Tyr1022). Anti-Phospho-JAK1 (Tyr1022) Polyclonal Antibody can be used in WB,IHC,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $217 | 7-10 days | |
100 μL | $317 | 7-10 days |
Description | Anti-Phospho-JAK1 (Tyr1022) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JAK1 (Tyr1022). Anti-Phospho-JAK1 (Tyr1022) Polyclonal Antibody can be used in WB,IHC,IF. |
Synonyms | p-JAK1 (Y1022), p-JAK1 (Tyr1022), JAK1 (p-Y1022), JAK1 (p-Tyr1022) |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western Blot analysis of various cells using Phospho-JAK1 (Y1034) Polyclonal Antibody diluted at 1:500. 2. Western blot analysis of lysates from A549 cells, using JAK1 (Phospho-Tyr1034) Antibody. The lane on the right is blocked with the phospho peptide. 3. Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,JAK1 (phospho Tyr1034) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2,Sodium citrate pH 6.0 was used for antibody retrieval(>98°C, 20min). 3,Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only. 4. Immunofluorescence analysis of mouse-liver tissue. 1,JAK1 (phospho Tyr1034) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2,Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B. |
Application | |
Recommended Dose | WB: 1:200-1000; IHC: 1:100-300; IF: 1:50-200 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Polyclonal Antibody |
Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Appearance | Liquid |
Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Research Background | Tyrosine kinase of the non-receptor type, involved in the IFN-a/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor. |
Conjucates | Unconjugated |
Others Formats | Phospho |
Immunogen | Peptide sequence around phosphorylation site of tyrosine 1034 (K-E-Y(p)-Y-T) derived from Human JAK1 |
Antigen Species | human |
Uniprot ID |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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