Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-Phospho-IRF3 (Ser386) Polyclonal Antibody

😃Good
Catalog No. TMAB-01434

Anti-Phospho-IRF3 (Ser386) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IRF3 (Ser386). Anti-Phospho-IRF3 (Ser386) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-Phospho-IRF3 (Ser386) Polyclonal Antibody

Anti-Phospho-IRF3 (Ser386) Polyclonal Antibody

😃Good
Catalog No. TMAB-01434
Anti-Phospho-IRF3 (Ser386) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IRF3 (Ser386). Anti-Phospho-IRF3 (Ser386) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$374 7-10 days
200 μL$529 7-10 days
Add to Cart
Add to Quotation
Bulk & Custom
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-Phospho-IRF3 (Ser386) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IRF3 (Ser386). Anti-Phospho-IRF3 (Ser386) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IRF3 (Ser386)) Polyclonal Antibody, Unconjugated (TMAB-01434) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IRF3 (Ser386)) Polyclonal Antibody, Unconjugated (TMAB-01434) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample: Bones (Mouse) Lysate at 40 μg
Primary: Anti-phospho-IRF3 (Ser386) (TMAB-01434) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
4. Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IRF3 (Ser386)) Polyclonal Antibody, Unconjugated (TMAB-01434) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IRF3 (Ser386)) Polyclonal Antibody, Unconjugated (TMAB-01434) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Blank control: A431. Primary Antibody (green line): Rabbit Anti-phospho-IRF3 (Ser386) antibody (TMAB-01434)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:50-200; FCM: 1ug/test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Note=Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011].
Antigen Details
Immunogen
KLH conjugated synthesised phosphopeptide: human IRF3 around the phosphorylation site of Ser386
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
SARS Coronavirus,TLR Signaling,Interferons,Other factors
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: buy Anti-Phospho-IRF3 (Ser386) Polyclonal Antibody | purchase Anti-Phospho-IRF3 (Ser386) Polyclonal Antibody | Anti-Phospho-IRF3 (Ser386) Polyclonal Antibody cost | order Anti-Phospho-IRF3 (Ser386) Polyclonal Antibody