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Anti-Phospho-Insulin Receptor (Tyr1361) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Insulin Receptor (Tyr1361). Anti-Phospho-Insulin Receptor (Tyr1361) Polyclonal Antibody can be used in WB,IHC,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $318 | 7-10 days |
Description | Anti-Phospho-Insulin Receptor (Tyr1361) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Insulin Receptor (Tyr1361). Anti-Phospho-Insulin Receptor (Tyr1361) Polyclonal Antibody can be used in WB,IHC,IF. |
Synonyms | p-Insulin Receptor (Y1361), p-Insulin Receptor (Tyr1361), Insulin Receptor (p-Y1361), Insulin Receptor (p-Tyr1361) |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of Insulin Receptor (phospho-Tyr1361) expression in THP1, HEK293T whole cell lysates. 2. Immunohistochemical analysis of Insulin Receptor (phospho-Tyr1361) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 3. Immunofluorescent analysis of Insulin Receptor (phospho-Tyr1361) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). |
Application | |
Recommended Dose | WB: 1:500-1000; IHC: 1:50-100; IF:1:100-300 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Polyclonal Antibody |
Purification | The antibody was purified by immunogen affinity chromatography. |
Appearance | Liquid |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Conjucates | Unconjugated |
Others Formats | Phospho |
Immunogen | KLH conjugated synthetic peptide: encompassing a sequence within the C-term region of human Insulin Receptor |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Theoretical: 95 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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