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Anti-Phospho-ILK-1 (Ser259) Polyclonal Antibody
Catalog No. TMAB-10859 Copy Product Info
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Anti-Phospho-ILK-1 (Ser259) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ILK-1 (Ser259). Anti-Phospho-ILK-1 (Ser259) Polyclonal Antibody can be used in WB.
Anti-Phospho-ILK-1 (Ser259) Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-10859
Anti-Phospho-ILK-1 (Ser259) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ILK-1 (Ser259). Anti-Phospho-ILK-1 (Ser259) Polyclonal Antibody can be used in WB.
Anti-Phospho-ILK-1
(Ser259) Polyclonal Antibody
(Ser259) Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-Phospho-ILK-1 (Ser259) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ILK-1 (Ser259). Anti-Phospho-ILK-1 (Ser259) Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | Sample: A431 (Human) Cell Lysate at 30 μg Primary: Anti-phospho-ILK-1 (Ser259) (TMAB-10859) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. |
| Tissue Specificity | Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The ILK protein is important in different biological pathways such as cell adhesion, anchorage-dependent cell cycle progression, oncogenic transformation, and growth factor signaling. The kinase activity of ILK is low in non-activated cells; its activity is stimulated by cell-ECM interactions and by certain growth factors. 3 Negative regulation of ILK is mediated by two phosphatases: PTEN, a tumor suppressor lipid sphatase, and ILKAP, a PP2C protein phosphatase. In tumor cells that do not express PTEN protein, ILK is constitutively active. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human ILK-1 around the phosphorylation site of Ser259 |
| Antigen Species | Human |
| Gene Name | ILK |
| Gene ID | |
| Protein Name | Integrin-linked protein kinase |
| Uniprot ID | |
| Function | Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. |
| Molecular Weight | Theoretical: 50 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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