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Anti-Phospho-IL7R (Tyr449) Polyclonal Antibody
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Catalog No. TMAB-10857
Anti-Phospho-IL7R (Tyr449) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IL7R (Tyr449). Anti-Phospho-IL7R (Tyr449) Polyclonal Antibody can be used in WB,FCM.
Anti-Phospho-IL7R (Tyr449) Polyclonal Antibody
Anti-Phospho-IL7R (Tyr449) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-10857
Anti-Phospho-IL7R (Tyr449) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IL7R (Tyr449). Anti-Phospho-IL7R (Tyr449) Polyclonal Antibody can be used in WB,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-IL7R (Tyr449) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IL7R (Tyr449). Anti-Phospho-IL7R (Tyr449) Polyclonal Antibody can be used in WB,FCM. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse) |
| Verified Activity | 1. Sample: U20s Cell (Human) Lysate at 40 μg Primary: Anti-phospho-IL-7Ra (Tyr449) (TMAB-10857) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kD Observed band size: 55 kD 2. Blank control: Hela. Primary Antibody (green line): Rabbit Anti-CD127 antibody (TMAB-10857) Dilution: 2 μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1 μg /test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | WB: 1:500-2000; FCM: 2μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted and Cell membrane. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in the V(D)J recombination during lymphocyte development. This protein is also found to control the accessibility of the TCR gamma locus by STAT5 and histone acetylation. Knockout studies in mice suggested that blocking apoptosis is an essential function of this protein during differentiation and activation of T lymphocytes. The functional defects in this protein may be associated with the pathogenesis of the severe combined immunodeficiency (SCID). |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human IL-7Ra around the phosphorylation site of Tyr449 |
| Antigen Species | Human |
| Gene Name | IL7R |
| Gene ID | |
| Protein Name | Interleukin-7 receptor subunit alpha |
| Uniprot ID | |
| Function | Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP). |
| Molecular Weight | Theoretical: 48 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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