Anti-Phospho-IL-1R1 (Tyr496) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-IL-1R1 (Tyr496). Anti-Phospho-IL-1R1 (Tyr496) Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Dog,Pig,Horse)

1. Sample: Intestine (Mouse) Lysate at 40 μg
Primary: Anti-phospho-IL-1R1 (Tyr496) (TMAB-10856) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
2. Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-phospho-IL-1R1 (Tyr496) antibody (TMAB-10856), Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg /test.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human IL-1R1 around the phosphorylation site of Tyr496

Human

IL1R1

Interleukin-1 receptor type 1

Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex.