Anti-Phospho-IKKi (Ser172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IKKi (Ser172). Anti-Phospho-IKKi (Ser172) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Dog,Pig,Cow,Rabbit)

1. Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-phospho-IKKi (Ser172) Polyclonal Antibody, Unconjugated (TMAB-10854) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Spleen (Mouse) Lysate at 40 μg
Thymus (Mouse) Lysate at 40 μg
Primary: Anti-phospho-IKKi (Ser172) (TMAB-10854) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 71 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthesised phosphopeptide: human IKKi/IKKE around the phosphorylation site of Ser172

Human

IKBKE

Inhibitor of nuclear factor kappa-B kinase subunit epsilon

Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.