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Anti-Phospho-IKBKB (Tyr199) Polyclonal Antibody
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Catalog No. TMAB-01430
Anti-Phospho-IKBKB (Tyr199) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IKBKB (Tyr199). Anti-Phospho-IKBKB (Tyr199) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Phospho-IKBKB (Tyr199) Polyclonal Antibody
Anti-Phospho-IKBKB (Tyr199) Polyclonal Antibody
😃Good
Catalog No. TMAB-01430
Anti-Phospho-IKBKB (Tyr199) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IKBKB (Tyr199). Anti-Phospho-IKBKB (Tyr199) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-IKBKB (Tyr199) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IKBKB (Tyr199). Anti-Phospho-IKBKB (Tyr199) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Horse,Rabbit) |
| Verified Activity | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-phospho-IKK beta (Tyr199) Polyclonal Antibody, Unconjugated (TMAB-01430) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-phospho-IKK beta (Tyr199) Polyclonal Antibody, Unconjugated (TMAB-01430) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-IKK beta (Tyr199)) Polyclonal Antibody, Unconjugated (TMAB-01430) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-IKK beta (Tyr199)) Polyclonal Antibody, Unconjugated (TMAB-01430) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (mouse bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-IKK beta (Tyr199)) Polyclonal Antibody, Unconjugated (TMAB-01430) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | IHC-P,IHC-Fr,IF |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Membrane raft. Note=Colocalized with DPP4 in membrane rafts. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | IKK beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK beta has been shown to activate NF-kB and phosphorylate IKB alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK1. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human IKK beta around the phosphorylation site of Tyr199 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | IKK,Inflammatory mediators,IKK,TLR Signaling,Other Kinases,NFkB Pathway |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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