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Anti-Phospho-IKB epsilon (Ser22) Polyclonal Antibody
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Catalog No. TMAB-10840
Anti-Phospho-IKB epsilon (Ser22) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IKB epsilon (Ser22). Anti-Phospho-IKB epsilon (Ser22) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-IKB epsilon (Ser22) Polyclonal Antibody
Anti-Phospho-IKB epsilon (Ser22) Polyclonal Antibody
😃Good
Catalog No. TMAB-10840
Anti-Phospho-IKB epsilon (Ser22) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IKB epsilon (Ser22). Anti-Phospho-IKB epsilon (Ser22) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-IKB epsilon (Ser22) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IKB epsilon (Ser22). Anti-Phospho-IKB epsilon (Ser22) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Cow,Sheep) |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Tissue Specificity | Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). For some genes, activation requires NFKB interaction with other transcription factors, such as STAT, AP1 (JUN), and NFAT. |
| Immunogen | KLH conjugated synthesised phosphopeptide: human IKB epsilon around the phosphorylation site of Ser22 |
| Antigen Species | Human |
| Gene Name | NFKBIE |
| Gene ID | |
| Protein Name | NF-kappa-B inhibitor epsilon |
| Uniprot ID | |
| Function | Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. |
| Molecular Weight | Theoretical: 39 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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