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Anti-Phospho-IkB alpha/NFKBIA (Tyr42) Polyclonal Antibody
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Catalog No. TMAB-01429
Alias p-IkB alpha/NFKBIA (Y42), p-IkB alpha/NFKBIA (Tyr42), nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α, IkB alpha/NFKBIA (p-Y42), IkB alpha/NFKBIA (p-Tyr42)
Anti-Phospho-IkB alpha/NFKBIA (Tyr42) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IkB alpha/NFKBIA (Tyr42). Anti-Phospho-IkB alpha/NFKBIA (Tyr42) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-Phospho-IkB alpha/NFKBIA (Tyr42) Polyclonal Antibody
Anti-Phospho-IkB alpha/NFKBIA (Tyr42) Polyclonal Antibody
😃Good
Catalog No. TMAB-01429Alias p-IkB alpha/NFKBIA (Y42), p-IkB alpha/NFKBIA (Tyr42), nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α, IkB alpha/NFKBIA (p-Y42), IkB alpha/NFKBIA (p-Tyr42)
Anti-Phospho-IkB alpha/NFKBIA (Tyr42) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IkB alpha/NFKBIA (Tyr42). Anti-Phospho-IkB alpha/NFKBIA (Tyr42) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-IkB alpha/NFKBIA (Tyr42) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IkB alpha/NFKBIA (Tyr42). Anti-Phospho-IkB alpha/NFKBIA (Tyr42) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Alias | p-IkB alpha/NFKBIA (Y42), p-IkB alpha/NFKBIA (Tyr42), nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α, IkB alpha/NFKBIA (p-Y42), IkB alpha/NFKBIA (p-Tyr42) |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Pig,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Blank control (blue line): Jurkat (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-IKB alpha (Tyr42) antibody (TMAB-01429), Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg/test. 2. Blank control (blue line): Hela (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C). Primary Antibody (green line): Rabbit Anti-phospho-IKB alpha (Tyr42) antibody (TMAB-01429), Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg/test. 3. Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 μg Lane 2: Hela (Human) Cell Lysate at 30 μg Primary: Anti-phospho-IKB alpha (Tyr42) (TMAB-01429) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kDa Observed band size: 37 kDa 4. Sample: Thymus (Mouse) Lysate at 40 μg Primary: Anti-phospho-IKB alpha (Tyr42) (TMAB-01429) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa 5. Sample: Raji (Human) Lysate at 40 μg Primary: Anti-phospho-IKB alpha (Tyr42) (TMAB-01429) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa 6. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-IKB alpha (Tyr42)) Polyclonal Antibody, Unconjugated (TMAB-01429) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human IKB alpha around the phosphorylation site of Tyr42 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | NFkB pathway,NFkB pathway,TLR Signaling,NFkB Pathway |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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