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Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody
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Catalog No. TMAB-01427
Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IkB alpha/NFKBIA (Ser36). Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody
Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody
😃Good
Catalog No. TMAB-01427
Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IkB alpha/NFKBIA (Ser36). Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IkB alpha/NFKBIA (Ser36). Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Pig,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Sample: Spleen (Mouse) Lysate at 40 μg BV2 (Mouse) Lysate at 30 μg Primary: Anti-phospho-IKB alpha (Ser36) (TMAB-01427) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kDa Observed band size: 35 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-IKB alpha (Ser36)) Polyclonal Antibody, Unconjugated (TMAB-01427) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-IKB alpha (Ser36)) polyclonal Antibody, Unconjugated (TMAB-01427) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
| Application | WB,IHC-P,IHC-Fr,ICC/IF,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human IKB alpha around the phosphorylation site of Ser36 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | NFkB pathway,NFkB pathway,TLR Signaling,NFkB Pathway |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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