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Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody

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Catalog No. TMAB-01427
Alias p-IkB alpha/NFKBIA (Ser36), p-IkB alpha/NFKBIA (S36), nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α, IkB alpha/NFKBIA (p-Ser36), IkB alpha/NFKBIA (p-S36)

Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IkB alpha/NFKBIA (Ser36). Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody

Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody

😃Good
Catalog No. TMAB-01427Alias p-IkB alpha/NFKBIA (Ser36), p-IkB alpha/NFKBIA (S36), nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α, IkB alpha/NFKBIA (p-Ser36), IkB alpha/NFKBIA (p-S36)
Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IkB alpha/NFKBIA (Ser36). Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IkB alpha/NFKBIA (Ser36). Anti-Phospho-IkB alpha/NFKBIA (Ser36) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Aliasp-IkB alpha/NFKBIA (Ser36), p-IkB alpha/NFKBIA (S36), nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α, IkB alpha/NFKBIA (p-Ser36), IkB alpha/NFKBIA (p-S36)
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Pig,Cow,Rabbit,Sheep)
Verified Activity
1. Sample:
Spleen (Mouse) Lysate at 40 μg
BV2 (Mouse) Lysate at 30 μg
Primary: Anti-phospho-IKB alpha (Ser36) (TMAB-01427) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-IKB alpha (Ser36)) Polyclonal Antibody, Unconjugated (TMAB-01427) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-IKB alpha (Ser36)) polyclonal Antibody, Unconjugated (TMAB-01427) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human IKB alpha around the phosphorylation site of Ser36
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
NFkB pathway,NFkB pathway,TLR Signaling,NFkB Pathway
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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