Anti-Phospho-IkB alpha/NFKBIA (Ser32) Polyclonal Antibody is a Rabbit antibody targeting Phospho-IkB alpha/NFKBIA (Ser32). Anti-Phospho-IkB alpha/NFKBIA (Ser32) Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Mouse,Rat (predicted:Pig,Cow,Rabbit,Sheep)

1. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-IKB alpha (Ser32)) polyclonal Antibody, Unconjugated (TMAB-01423) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-IKB alpha (Ser32)) Polyclonal Antibody, Unconjugated (TMAB-01423) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-IKB alpha (Ser32)) Polyclonal Antibody, Unconjugated (TMAB-01423) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IHC-P,IHC-Fr,ICC/IF,IF

IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human IKB alpha around the phosphorylation site of Ser32

Human

NFkB pathway,NFkB pathway,TLR Signaling,NFkB Pathway