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Anti-Phospho-IkB alpha/NFKBIA (Ser32, 36) Antibody (7S693)
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Catalog No. TMAB-01425
Anti-Phospho-IkB alpha/NFKBIA (Ser32, 36) Antibody (7S693) is a Rabbit antibody targeting Phospho-IkB alpha/NFKBIA (Ser32, 36). Anti-Phospho-IkB alpha/NFKBIA (Ser32, 36) Antibody (7S693) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-IkB alpha/NFKBIA (Ser32, 36) Antibody (7S693)
Anti-Phospho-IkB alpha/NFKBIA (Ser32, 36) Antibody (7S693)
😃Good
Catalog No. TMAB-01425
Anti-Phospho-IkB alpha/NFKBIA (Ser32, 36) Antibody (7S693) is a Rabbit antibody targeting Phospho-IkB alpha/NFKBIA (Ser32, 36). Anti-Phospho-IkB alpha/NFKBIA (Ser32, 36) Antibody (7S693) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μL | $154 | 7-10 days | |
| 50 μL | $271 | 7-10 days | |
| 100 μL | $489 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat |
| Ig Type | IgG |
| Clone | 7S693 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Blocking buffer: 5% NFDM/TBST Primary dilution: 1:2000 Primary incubation condition: 2 hours at room temperature Secondary: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): HeLa, (+): HeLa+ Human α-TNF (20ng/ml, 30 min) Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 36 kDa Observed MW: 36 kDa 2. Blocking buffer: 5% NFDM/TBST Primary dilution: 1:2000 Primary incubation condition: 2 hours at room temperature Secondary: Goat Anti-Rabbit IgG H&L (HRP) Lysate: (-): C6, (+): C6+ Calyculin A (100nM, 30 min) Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 36 kDa Observed MW: 36 kDa 3. Tissue: (A) Rat kidney, (B) Rat kidney, then the tissue was incubated with alkaline phosphatase (50 U/ml) at 37°C for 1 hour Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary dilution: 1:100 Primary incubation condition: 1 hour at room temperature Secondary: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-01425 4. Tissue: (A) Mouse kidney, (B) Mouse kidney, then the tissue was incubated with alkaline phosphatase (50 U/ml) at 37°C for 1 hour Section type: Formalin-fixed & Paraffin-embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary dilution: 1:100 Primary incubation condition: 1 hour at room temperature Secondary: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for TMAB-01425 |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:50-100; IF: 1:1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| Immunogen | KLH conjugated synthetic peptide: human phospho-IKB Alpha (Ser32 / Ser36) |
| Antigen Species | Human |
| Gene ID | 4792 |
| Uniprot ID | |
| Biology Area | NFkB pathway,NFkB pathway,TLR Signaling,NFkB Pathway |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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