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Anti-Phospho-IFNGR1 (Tyr457) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-IFNGR1 (Tyr457). Anti-Phospho-IFNGR1 (Tyr457) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
| Description | Anti-Phospho-IFNGR1 (Tyr457) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-IFNGR1 (Tyr457). Anti-Phospho-IFNGR1 (Tyr457) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-IFNGR1) Polyclonal Antibody, Unconjugated (TMAB-10837) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 2. Sample: Jurkat (Human) Cell Lysate at 40 μg Primary: Anti-p-IFNGR1 (TMAB-10837) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD 3. Sample: Panc-1 (Human) Cell Lysate at 40 μg A431 (Human) Cell Lysate at 40 μg Primary: Anti-p-IFNGR1 (TMAB-10837) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD 4. Sample: 293T Cell (Human) Lysate at 40 μg Primary: Anti-phospho-IFNGR1 (Tyr457) (TMAB-10837) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene (IFNGR1) encodes the ligand-binding chain(alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthesised phosphopeptide: human IFNGR1 around the phosphorylation site of Tyr457 |
| Antigen Species | Human |
| Gene Name | IFNGR1 |
| Gene ID | |
| Protein Name | Interferon gamma receptor 1 |
| Uniprot ID | |
| Function | Receptor for interferon gamma. Two receptors bind one interferon gamma dimer. |
| Molecular Weight | Theoretical: 53 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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