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Anti-Phospho-IFNGR1 (Tyr457) Polyclonal Antibody 2

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Catalog No. TMAB-10837

Anti-Phospho-IFNGR1 (Tyr457) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-IFNGR1 (Tyr457). Anti-Phospho-IFNGR1 (Tyr457) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Phospho-IFNGR1 (Tyr457) Polyclonal Antibody 2

Anti-Phospho-IFNGR1 (Tyr457) Polyclonal Antibody 2

Copy Product Info
😃Good
Catalog No. TMAB-10837
Anti-Phospho-IFNGR1 (Tyr457) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-IFNGR1 (Tyr457). Anti-Phospho-IFNGR1 (Tyr457) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5297-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Phospho-IFNGR1 (Tyr457) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-IFNGR1 (Tyr457). Anti-Phospho-IFNGR1 (Tyr457) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-IFNGR1) Polyclonal Antibody, Unconjugated (TMAB-10837) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Sample: Jurkat (Human) Cell Lysate at 40 μg
Primary: Anti-p-IFNGR1 (TMAB-10837) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
3. Sample:
Panc-1 (Human) Cell Lysate at 40 μg
A431 (Human) Cell Lysate at 40 μg
Primary: Anti-p-IFNGR1 (TMAB-10837) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
4. Sample: 293T Cell (Human) Lysate at 40 μg
Primary: Anti-phospho-IFNGR1 (Tyr457) (TMAB-10837) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single-pass type I membrane protein.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene (IFNGR1) encodes the ligand-binding chain(alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection. [provided by RefSeq].
Antigen Details
Immunogen
KLH conjugated synthesised phosphopeptide: human IFNGR1 around the phosphorylation site of Tyr457
Antigen Species
Human
Gene Name
IFNGR1
Gene ID
Protein Name
Interferon gamma receptor 1
Uniprot ID
Function
Receptor for interferon gamma. Two receptors bind one interferon gamma dimer.
Chemical Properties
Molecular WeightTheoretical: 53 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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