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Anti-Phospho-ICAM-1/CD54 (Tyr512) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ICAM-1/CD54 (Tyr512). Anti-Phospho-ICAM-1/CD54 (Tyr512) Polyclonal Antibody can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $218 | 7-10 days | |
100 μL | $318 | 7-10 days |
Description | Anti-Phospho-ICAM-1/CD54 (Tyr512) Polyclonal Antibody is a Rabbit antibody targeting Phospho-ICAM-1/CD54 (Tyr512). Anti-Phospho-ICAM-1/CD54 (Tyr512) Polyclonal Antibody can be used in WB. |
Synonyms | p-ICAM-1/CD54 (Y512), p-ICAM-1/CD54 (Tyr512), ICAM-1/CD54 (p-Y512), ICAM-1/CD54 (p-Tyr512) |
Ig Type | IgG |
Reactivity | Human |
Verified Activity | 1. Western blot analysis of extracts from HUVEC cells using ICAM-1 (Phospho-Tyr512) Antibody TMAC-02083 (Lane 2) and the same antibody preincubated with blocking peptide (Lane1). 2. Western blot analysis of extracts from C2C12 cells, treated with starve or calf intestinal phosphatase (CIP), using ICAM-1 (Phospho-Tyr512) Antibody TMAC-02083. |
Application | |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Polyclonal Antibody |
Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
Appearance | Liquid |
Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin a-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. |
Conjucates | Unconjugated |
Others Formats | Phospho |
Immunogen | Peptide sequence around phosphorylation site of tyrosine 512 (K-K-Y(p)-R-L) derived from Human ICAM-1 |
Antigen Species | human |
Uniprot ID |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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