Anti-Phospho-Hsp90 beta (Ser226) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Hsp90 beta (Ser226). Anti-Phospho-Hsp90 beta (Ser226) Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat)

1. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-phospho-Hsp90 beta (Ser226) antibody (TMAB-10834)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Blank control (blue line): A549 (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-phospho-Hsp90 beta (Ser226) antibody (TMAB-10834), Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg /test.

FCM: 1μg/Test

Polyclonal

KLH conjugated synthesised phosphopeptide: human Hsp90 beta around the phosphorylation site of Ser226

Human

HSP90AB1

Heat shock protein HSP 90-beta

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.