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Anti-Phospho-HSF1 (Ser326) Antibody (5K398)
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Catalog No. TMAB-10820
Anti-Phospho-HSF1 (Ser326) Antibody (5K398) is a Rabbit antibody targeting Phospho-HSF1 (Ser326). Anti-Phospho-HSF1 (Ser326) Antibody (5K398) can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-Phospho-HSF1 (Ser326) Antibody (5K398)
Anti-Phospho-HSF1 (Ser326) Antibody (5K398)
😃Good
Catalog No. TMAB-10820
Anti-Phospho-HSF1 (Ser326) Antibody (5K398) is a Rabbit antibody targeting Phospho-HSF1 (Ser326). Anti-Phospho-HSF1 (Ser326) Antibody (5K398) can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 200 μg | $1,059 | 7-10 days | 7-10 days | |
| 25 μL | $151 | 7-10 days | 7-10 days | |
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days | |
| 200 μL | $755 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-HSF1 (Ser326) Antibody (5K398) is a Rabbit antibody targeting Phospho-HSF1 (Ser326). Anti-Phospho-HSF1 (Ser326) Antibody (5K398) can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
| Ig Type | IgG |
| Clone | 5K398 |
| Reactivity | Human,Mouse (predicted:Rat) |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2μg/Test |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic duringnormal growth. On activation, translocates to nuclear stressgranules. Colocalizes with SUMO1 in nuclear stress granules. |
| Tissue Specificity | Phosphorylated on multiple serine residues, a subset of whichare involved in stress-related regulation of transcriptionactivation. Constitutive phosphorylation represses transcriptionalactivity at normal temperatures. Levels increase on specificresidues heat-shock and enhance HSF1 transactivation activity.Phosphorylation on Ser-307 derepresses activation on heat-stressand in combination with Ser-303 phosphorylation appears to beinvolved in recovery after heat-stress. Phosphorylated on Ser-230by CAMK2, in vitro. Cadmium also enhances phosphorylation at thissite. Phosphorylation on Ser-303 is a prerequisite for HSF1sumoylation. Phosphorylation on Ser-121 inhibits transactivationand promotes HSP90 binding. Phosphorylation on Thr-142 alsomediates transcriptional activity induced by heat. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human HSF1 around the phosphorylation site of Ser326 |
| Antigen Species | Human |
| Gene Name | HSF1 |
| Gene ID | |
| Protein Name | Heat shock factor protein 1 |
| Uniprot ID | |
| Function | DNA-binding protein that specifically binds heat shockpromoter elements (HSE) and activates transcription. In highereukaryotes, HSF is unable to bind to the HSE unless the cells areheat shocked. |
| Molecular Weight | Theoretical: 57 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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