Anti-Phospho-HOMER3 (Thr36) Polyclonal Antibody is a Rabbit antibody targeting Phospho-HOMER3 (Thr36). Anti-Phospho-HOMER3 (Thr36) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog)

1. Sample: A549 Cell (Human) Lysate at 40 μg
Primary: Anti-phospho-HOMER3 (Thr36) (TMAB-10811) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD
2. Sample: U937 (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-HOMER3 (Thr36) (TMAB-10811) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD
3. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HOMER3 (Thr36)) Polyclonal Antibody, Unconjugated (TMAB-10811) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human HOMER3 around the phosphorylation site of Thr36

Human

HOMER3

Homer protein homolog 3

Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses.