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Anti-Phospho-HNRPD (Ser83) Polyclonal Antibody is a Rabbit antibody targeting Phospho-HNRPD (Ser83). Anti-Phospho-HNRPD (Ser83) Polyclonal Antibody can be used in IF,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $218 | 7-10 days | 7-10 days | |
| 100 μL | $316 | 7-10 days | 7-10 days |
| Description | Anti-Phospho-HNRPD (Ser83) Polyclonal Antibody is a Rabbit antibody targeting Phospho-HNRPD (Ser83). Anti-Phospho-HNRPD (Ser83) Polyclonal Antibody can be used in IF,IHC,WB. |
| Synonyms | p-HNRPD (Ser83), p-HNRPD (S83), HNRPD (p-Ser83), HNRPD (p-S83) |
| Ig Type | IgG |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Western blot analysis of extracts from JK cells (Lane 2), using hnRPD (Phospho-Ser83) Antibody TMAC-02001. The lane on the left is treated with antigen-specific peptide. 2. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using hnRPD (Phospho-Ser83) antibody TMAC-02001 (left)or the same antibody preincubated with blocking peptide (right). 3. Immunofluorescence staining of methanol-fixed HeLa cells using hnRPD (Phospho-Ser83) Antibody TMAC-02001. ![]() ![]() ![]() |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-100; IF: 1:100-200 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of Serine 83(N-S-S(p)-P-R) derived from Human hnRPD |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Actual: 38 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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