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Anti-Phospho-Histone H3 (Ser10) Antibody (5C369)
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Catalog No. TMAY-01536
Alias Phospho-Histone H3 (S10), p-Histone H3 (Ser10), p-Histone H3 (S10), Histone H3 (p-Ser10), Histone H3 (p-S10)
Anti-Phospho-Histone H3 (Ser10) Antibody (5C369) is a Rabbit antibody targeting Phospho-Histone H3 (Ser10). Anti-Phospho-Histone H3 (Ser10) Antibody (5C369) can be used in WB.
Anti-Phospho-Histone H3 (Ser10) Antibody (5C369)
Anti-Phospho-Histone H3 (Ser10) Antibody (5C369)
😃Good
Catalog No. TMAY-01536Alias Phospho-Histone H3 (S10), p-Histone H3 (Ser10), p-Histone H3 (S10), Histone H3 (p-Ser10), Histone H3 (p-S10)
Anti-Phospho-Histone H3 (Ser10) Antibody (5C369) is a Rabbit antibody targeting Phospho-Histone H3 (Ser10). Anti-Phospho-Histone H3 (Ser10) Antibody (5C369) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $82 | 7-10 days | |
| 100 μL | $233 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB Reactivity: Human |
| Alias | Phospho-Histone H3 (S10), p-Histone H3 (Ser10), p-Histone H3 (S10), Histone H3 (p-Ser10), Histone H3 (p-S10) |
| Ig Type | Rabbit IgG |
| Clone | 5C369 |
| Reactivity | Human;
Species predicted to react based on 100% sequence homology: mouse, rat |
| Verified Activity | 1. Western blot analysis of extracts from serum-starved Hela, untreated (-) or treated with Calyculin A (100 nM, 30 min; +), using Phospho-Histone H3 (Ser10)rabbit monoclonal Antibody at 1:10000 dilution. 2. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with calyculin A (100 nM, 30min; +) (line B); treated with calyculin A and λ-phosphatase (line C) using Phospho-Histone H3 (Ser10) rabbit monoclonal Antibody at 1:20000 dilution. (Validation Experiment) 3. Western blot analysis of extracts from serum-starved Hela, untreated (line A); treated with calyculin A (100 nM, 30min; +) (line B), using Phospho-Histone H3 (Ser10) rabbit monoclonal Antibody at 1:20000 dilution (upper) or anti-Histone H3 antibody (lower). (Validation Experiment) |
| Application | WB |
| Recommended Dose | WB: 1:10000-1:50000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to residues around Ser10 of Human Phospho-Histone H3. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| Research Background | Histone H3.1, also known as HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, is a member of the histone H3 family which is a core component of nucleosome. It is expressed during the S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher-order chromatin structures. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide corresponding to residues around Ser10 of Human Phospho-Histone H3 |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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