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Anti-Phospho-Histone H2A.X (Ser139) Antibody (6R272)
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Catalog No. TMAY-01424
Alias Phospho-Histone H2A.X (S139), p-Histone H2A.X (Ser139), p-Histone H2A.X (S139), Histone H2A.X (p-Ser139), Histone H2A.X (p-S139)
Anti-Phospho-Histone H2A.X (Ser139) Antibody (6R272) is a Rabbit antibody targeting Phospho-Histone H2A.X (Ser139). Anti-Phospho-Histone H2A.X (Ser139) Antibody (6R272) can be used in WB,IHC-P.
Anti-Phospho-Histone H2A.X (Ser139) Antibody (6R272)
Anti-Phospho-Histone H2A.X (Ser139) Antibody (6R272)
😃Good
Catalog No. TMAY-01424Alias Phospho-Histone H2A.X (S139), p-Histone H2A.X (Ser139), p-Histone H2A.X (S139), Histone H2A.X (p-Ser139), Histone H2A.X (p-S139)
Anti-Phospho-Histone H2A.X (Ser139) Antibody (6R272) is a Rabbit antibody targeting Phospho-Histone H2A.X (Ser139). Anti-Phospho-Histone H2A.X (Ser139) Antibody (6R272) can be used in WB,IHC-P.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $82 | 7-10 days | |
| 100 μL | $235 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, IHC-P Reactivity: Human |
| Alias | Phospho-Histone H2A.X (S139), p-Histone H2A.X (Ser139), p-Histone H2A.X (S139), Histone H2A.X (p-Ser139), Histone H2A.X (p-S139) |
| Ig Type | Rabbit IgG |
| Clone | 6R272 |
| Reactivity | Human;
Predicted to React with:>Phospho-Histone H2A.X (Ser139). Also named as gamma H2AX, γ H2AX.Phospho-Histone H2A.X Ser140 of P16104 in UniProt historically has been referenced as Ser139. |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue, untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-Histone H2A-X (Ser139) Antibody, Rabbit Monoclonal at 1:200 dilution. 2. Western blot analysis of extracts from serum-starved NIH-3T3, untreated(line A) or treated with UV (30min; +)(line B), using Phospho-Histone H2A.X (Ser139) rabbit monoclonal Antibody at 1:5000 dilution (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution (lower). 3. Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with UV (30min; +) (line B); treated with UV and λ-phosphatase (line C) using Phospho-Histone H2A.X (Ser139) rabbit monoclonal Antibody at 1:5000 dilution. (Validation Experiment) 4. Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with UV (30min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-Histone H2A.X (Ser139) rabbit monoclonal Antibody at 1:5000 dilution. (Validation Experiment) |
| Application | WB,IHC-P |
| Recommended Dose | WB: 1:2000-1:20000; IHC-P: 1:1000-1:5000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to residues around Ser139 of Human Phospho-Histone H2A.X. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5 |
| Research Background | H2AFX variants are associated with an increased risk of breast cancer (BC). H2AFX gene encodes a member of the H2A histone family which is central in the detection of and response to DNA double-strand breaks. In somatic cells, H2afx and Mdc1 are close functional partners in DNA repair and damage response. Due to the critical role of the H2AX histone variant in double-strand break repair, genetic variants in the H2AX gene, H2AFX, may influence cancer susceptibility. H2AFX encodes a histone variant involved in signaling sites of DNA damage and recruiting repair factors. Genetic variants in H2AFX may influence the risk of non-Hodgkin lymphoma (NHL), a heterogeneous group of lymphoid tumors that are characterized by chromosomal translocations. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: residues around Ser139 of Human Phospho-Histone H2A.X |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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