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Anti-Phospho-HIST1H3A (Ser10) Polyclonal Antibody is a Rabbit antibody targeting Phospho-HIST1H3A (Ser10). Anti-Phospho-HIST1H3A (Ser10) Polyclonal Antibody can be used in IF,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $216 | 7-10 days | 7-10 days | |
| 100 μL | $317 | 7-10 days | 7-10 days |
| Description | Anti-Phospho-HIST1H3A (Ser10) Polyclonal Antibody is a Rabbit antibody targeting Phospho-HIST1H3A (Ser10). Anti-Phospho-HIST1H3A (Ser10) Polyclonal Antibody can be used in IF,IHC,WB. |
| Synonyms | p-HIST1H3A (Ser10), p-HIST1H3A (S10), HIST1H3A (p-Ser10), HIST1H3A (p-S10) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Histone H3.1 (Phospho-Ser10) Antibody TMAC-01949 (left) or the same antibody preincubated with blocking peptide (right). 2. Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using Histone H3.1 (Phospho-Ser10) Antibody TMAC-01949. 3. Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 (Phospho-Ser10) Antibody TMAC-01949. 4. Immunofluorescence staining of methanol-fixed Hela cells using Histone H3.1 (Phospho-Ser10) Antibody TMAC-01949. 5. Western blot analysis of extracts of various cell lines, using Histone H3.1(Phospho-Ser10) Antibody TMAC-01949 ![]() ![]() ![]() ![]() ![]() |
| Application | |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of serine 10 (R-K-S(p)-T-G) derived from Human Histone H31 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Actual: 17 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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