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Anti-Phospho-HEF1 (Ser369) Polyclonal Antibody
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Catalog No. TMAB-10778
Anti-Phospho-HEF1 (Ser369) Polyclonal Antibody is a Rabbit antibody targeting Phospho-HEF1 (Ser369). Anti-Phospho-HEF1 (Ser369) Polyclonal Antibody can be used in WB.
Anti-Phospho-HEF1 (Ser369) Polyclonal Antibody
Anti-Phospho-HEF1 (Ser369) Polyclonal Antibody
😃Good
Catalog No. TMAB-10778
Anti-Phospho-HEF1 (Ser369) Polyclonal Antibody is a Rabbit antibody targeting Phospho-HEF1 (Ser369). Anti-Phospho-HEF1 (Ser369) Polyclonal Antibody can be used in WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-HEF1 (Ser369) Polyclonal Antibody is a Rabbit antibody targeting Phospho-HEF1 (Ser369). Anti-Phospho-HEF1 (Ser369) Polyclonal Antibody can be used in WB. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Cow,Horse,Sheep) |
| Application | |
| Recommended Dose | WB: 1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cell cortex. Nucleus. Golgi apparatus. Cell projection, lamellipodium. Cytoplasm. Cell junction, focal adhesion. Note=Localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with mitotic spindle. Enhancer of filamentation 1 p55: Cytoplasm, cytoskeleton, spindle. |
| Tissue Specificity | Widely expressed. Higher levels detected in kidney, lung, and placenta. Also detected in T-cells, B-cells and diverse cell lines. The protein has been detected in lymphocytes, in diverse cell lines, and in lung tissues. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form. |
| Immunogen | KLH conjugated synthesised phosphopeptide: human HEF1 around the phosphorylation site of Ser369 |
| Antigen Species | Human |
| Gene Name | NEDD9 |
| Gene ID | |
| Protein Name | Enhancer of filamentation 1 |
| Uniprot ID | |
| Function | Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form. |
| Molecular Weight | Theoretical: 93 kDa. |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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