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Anti-Phospho-H2AFX (Ser139) Polyclonal Antibody 2
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Catalog No. TMAC-01852
Alias p-H2AFX (Ser139), p-H2AFX (S139), H2AFX (p-Ser139), H2AFX (p-S139)
Anti-Phospho-H2AFX (Ser139) Polyclonal Antibody is a Rabbit antibody targeting Phospho-H2AFX (Ser139). Anti-Phospho-H2AFX (Ser139) Polyclonal Antibody can be used in IF,WB.
Anti-Phospho-H2AFX (Ser139) Polyclonal Antibody 2
Anti-Phospho-H2AFX (Ser139) Polyclonal Antibody 2
😃Good
Catalog No. TMAC-01852Alias p-H2AFX (Ser139), p-H2AFX (S139), H2AFX (p-Ser139), H2AFX (p-S139)
Anti-Phospho-H2AFX (Ser139) Polyclonal Antibody is a Rabbit antibody targeting Phospho-H2AFX (Ser139). Anti-Phospho-H2AFX (Ser139) Polyclonal Antibody can be used in IF,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $216 | 7-10 days | 7-10 days | |
| 100 μL | $316 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Phospho-H2AFX (Ser139) Polyclonal Antibody is a Rabbit antibody targeting Phospho-H2AFX (Ser139). Anti-Phospho-H2AFX (Ser139) Polyclonal Antibody can be used in IF,WB. |
| Synonyms | p-H2AFX (Ser139), p-H2AFX (S139), H2AFX (p-Ser139), H2AFX (p-S139) |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of extracts from HT29 cells untreated (lane 1) or treated with UV (lane 2) using Histone H2A.X (Phospho-Ser139) Antibody TMAC-01852. 2. Immunofluorescence staining of methanol-fixed Hela cells using Histone H2A.X (Phospho-Ser139) Antibody TMAC-01852. 3. Western blot analysis of extracts from 293 cells, treated with UV+serum or calf intestinal phosphatase (CIP), using Histone H2A.X (Phospho-Ser139) Antibody TMAC-01852.    |
| Application | |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Appearance | Liquid |
| Formulation | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. |
| Conjucates | Unconjugated |
| Others Formats | Phospho |
| Immunogen | Peptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Human Histone H2AX |
| Antigen Species | human |
| Uniprot ID |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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