Anti-Phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) Antibody (2N419) is a Rabbit antibody targeting Phospho-GSK3 Alpha + Beta (Tyr279+Tyr216). Anti-Phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) Antibody (2N419) can be used in WB.

IgG

2N419

Human,Mouse,Rat

Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Testis tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Testis tissue lysates
Lane 5: Human HeLa cell lysates
Lane 6: Human 293T cell lysates
Lane 7: Human HepG2 cell lysates
Lane 8: Human MCF-7 cell lysates
Primary: Anti-phospho-GSK3 Alpha+Beta (Tyr279+Tyr216) (TMAB-10757) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47/51 kDa
Observed band size: 47,51 kDa

WB: 1:500-2000

Monoclonal

KLH conjugated Synthesised phosphopeptide: human GSK-3 Beta around the phosphorylation site of Tyr216

Human

GSK3A

Glycogen synthase kinase-3 alpha

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transctiption factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth.