Anti-Phospho-Gria2 (Ser880) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-Gria2 (Ser880). Anti-Phospho-Gria2 (Ser880) Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Cow,Horse)

1. Sample:
Lane 1: Mouse Cerebellum tissue lysates
Lane 2: Rat Cerebellum tissue lysates
Primary: Anti-phospho-Gria2 (Ser880) (TMAB-10752) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 96 kDa
Observed band size: 138 kDa
2. Paraformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (phospho-Gria2 (Ser880)) Polyclonal Antibody, Unconjugated (TMAB-10752) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human Gria2 around the phosphorylation site of Ser880

Human

GRIA2

Glutamate receptor 2

Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.