Anti-Phospho-GluR1 (Ser849) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GluR1 (Ser849). Anti-Phospho-GluR1 (Ser849) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Sheep)

1. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Cerebellum (Mouse) Lysate at 40 μg
Primary: Anti-phospho-GluR1 (Ser849) (TMAB-10745) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 128 kD
2. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Cerebellum (Mouse) Lysate at 40 μg
U251 (Human) Lysate at 30 μg
Primary: Anti-phospho-GluR1 (Ser849) (TMAB-10745) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 128 kD
3. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-GluR1 (Ser849)) Polyclonal Antibody, Unconjugated (TMAB-10745) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Sample:
Brain (Mouse) Lysate at 40 μg
Cerebellum (Mouse) Lysate at 40 μg
Cerebellum (Rat) Lysate at 40 μg
U251 (Human) Lysate at 30 μg
Primary: Anti-phospho-GluR1 (Ser849) (TMAB-10745) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 130 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthesised phosphopeptide: human GluR1 around the phosphorylation site of Ser849

Human

GRIA1

Glutamate receptor 1

Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.