Anti-Phospho-GluR1 (Ser836) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GluR1 (Ser836). Anti-Phospho-GluR1 (Ser836) Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat)

Blank control: sH-SY5Y.
Primary Antibody (green line): Rabbit Anti-phospho-GluR1 (Ser836) antibody (TMAB-10744)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Polyclonal

KLH conjugated synthesised phosphopeptide: human GluR1 around the phosphorylation site of Ser836

Human

GRIA1

Glutamate receptor 1

Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.