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Anti-Phospho-GCN2 (Thr899) Polyclonal Antibody

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Catalog No. TMAB-01415
Alias p-GCN2 (Thr899), p-GCN2 (T899), MGCN2, KIAA1338, GCN2-like protein, GCN2 eIF2alpha kinase, GCN2 (p-Thr899), GCN2 (p-T899), GCN2, Eukaryotic Translation Initiation Factor 2-alpha kinase 4, Eukaryotic Translation Initiation Factor 2 alpha kinase 4, Eif2ak4, EIF2AK4, E2AK4

Anti-Phospho-GCN2 (Thr899) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GCN2 (Thr899). Anti-Phospho-GCN2 (Thr899) Polyclonal Antibody can be used in FCM.

Anti-Phospho-GCN2 (Thr899) Polyclonal Antibody

Anti-Phospho-GCN2 (Thr899) Polyclonal Antibody

😃Good
Catalog No. TMAB-01415Alias p-GCN2 (Thr899), p-GCN2 (T899), MGCN2, KIAA1338, GCN2-like protein, GCN2 eIF2alpha kinase, GCN2 (p-Thr899), GCN2 (p-T899), GCN2, Eukaryotic Translation Initiation Factor 2-alpha kinase 4, Eukaryotic Translation Initiation Factor 2 alpha kinase 4, Eif2ak4, EIF2AK4, E2AK4
Anti-Phospho-GCN2 (Thr899) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GCN2 (Thr899). Anti-Phospho-GCN2 (Thr899) Polyclonal Antibody can be used in FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3727-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-GCN2 (Thr899) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GCN2 (Thr899). Anti-Phospho-GCN2 (Thr899) Polyclonal Antibody can be used in FCM.
Aliasp-GCN2 (Thr899), p-GCN2 (T899), MGCN2, KIAA1338, GCN2-like protein, GCN2 eIF2alpha kinase, GCN2 (p-Thr899), GCN2 (p-T899), GCN2, Eukaryotic Translation Initiation Factor 2-alpha kinase 4, Eukaryotic Translation Initiation Factor 2 alpha kinase 4, Eif2ak4, EIF2AK4, E2AK4
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Dog,Cow,Horse)
Verified Activity
1. Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-Phospho-GCN2 (Thr899) antibody (TMAB-01415)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-Phospho-GCN2 (Thr899) antibody (TMAB-01415) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
FCM
Recommended Dose
FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundGCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses.
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human GCN2 around the phosphorylation site of Thr899
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Regulation,Other Kinases
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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