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Anti-Phospho-GCN2 (Thr899) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GCN2 (Thr899). Anti-Phospho-GCN2 (Thr899) Polyclonal Antibody can be used in FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-Phospho-GCN2 (Thr899) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GCN2 (Thr899). Anti-Phospho-GCN2 (Thr899) Polyclonal Antibody can be used in FCM. |
Alias | p-GCN2 (Thr899), p-GCN2 (T899), MGCN2, KIAA1338, GCN2-like protein, GCN2 eIF2alpha kinase, GCN2 (p-Thr899), GCN2 (p-T899), GCN2, Eukaryotic Translation Initiation Factor 2-alpha kinase 4, Eukaryotic Translation Initiation Factor 2 alpha kinase 4, Eif2ak4, EIF2AK4, E2AK4 |
Ig Type | IgG |
Reactivity | Human,Mouse (predicted:Rat,Dog,Cow,Horse) |
Verified Activity | 1. Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-Phospho-GCN2 (Thr899) antibody (TMAB-01415) Dilution: 2 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 2. Blank control (Black line): Molt4 (Black). Primary Antibody (green line): Rabbit Anti-Phospho-GCN2 (Thr899) antibody (TMAB-01415) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
Application | FCM |
Recommended Dose | FCM: 1ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses. |
Immunogen | KLH conjugated Synthesised phosphopeptide: human GCN2 around the phosphorylation site of Thr899 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Regulation,Other Kinases |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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