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Anti-Phospho-GCN2 (Thr667) Polyclonal Antibody
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Catalog No. TMAB-10733
Anti-Phospho-GCN2 (Thr667) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GCN2 (Thr667). Anti-Phospho-GCN2 (Thr667) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-GCN2 (Thr667) Polyclonal Antibody
Anti-Phospho-GCN2 (Thr667) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-10733
Anti-Phospho-GCN2 (Thr667) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GCN2 (Thr667). Anti-Phospho-GCN2 (Thr667) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $223 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Antigen Details
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| Description | Anti-Phospho-GCN2 (Thr667) Polyclonal Antibody is a Rabbit antibody targeting Phospho-GCN2 (Thr667). Anti-Phospho-GCN2 (Thr667) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Dog,Cow,Horse) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-GCN2 (Thr667)) Polyclonal Antibody, Unconjugated (TMAB-10733) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-GCN2 (Thr667)) Polyclonal Antibody, Unconjugated (TMAB-10733) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 3. Sample: Hippocampus (Mouse) Lysate at 40 μg Cerebral cortex (Mouse) Lysate at 40 μg Primary: Anti-phospho-GCN2 (Thr667) (TMAB-10733) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 181 kD Observed band size: 170 kD 4. Sample: Cerebrum (Mouse) Lysate at 40 μg Cerebrum (Rat) Lysate at 40 μg Hippocampus (Mouse) Lysate at 40 μg Cerebral cortex (Mouse) Lysate at 40 μg Primary: Anti-phospho-GCN2 (Thr667) (TMAB-10733) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 181 kD Observed band size: 170/181 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Tissue Specificity | Widely expressed. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human GCN2 around the phosphorylation site of Thr667 |
| Antigen Species | Human |
| Gene Name | EIF2AK4 |
| Gene ID | |
| Protein Name | eIF-2-alpha kinase GCN2 |
| Uniprot ID | |
| Function | Can phosphorylate the alpha subunit of EIF2 and may mediate translational control. |
| Molecular Weight | Theoretical: 181 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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